IRB Faculty
The Medical &
Scientific Research Directorate
Dr. Kwabena Frimpong-Manso Opuni
PhD
Senior Lecturer/Head, Department of Pharmaceutical Chemistry
kfopuni@ug.edu.gh | +233-208-260595 |
Education
- B. Pharm (Hons): Kwame Nkrumah University of Science & Technology, Faculty of Pharmacy & Pharmaceutical Sciences, Kumasi, Ghana (2001) ·
- Certificate, Management of Drug Supplies: Robert Gordon University, Aberdeen, Scotland (2002) ·
- MSc, Pharmaceutical Analysis & Quality Control: Kwame Nkrumah University of Science & Technology, Faculty of Pharmacy & Pharmaceutical Sciences, Kumasi, Ghana (2005)
- PhD, Analytical Chemistry / Proteomics (Summa cum laude): University of Rostock, Proteome Center Rostock, Rostock, Germany (2017)
- DAAD Postdoctoral Fellowship, Proteomics (Infectious Disease): Proteome Center Rostock, Rostock, Germany (May to July 2019)
Board Certifications
- Registered Pharmacist, Pharmacy Council Ghana
- Project Management Professional, Project Management Institute, USA
Research Interest
My research focus on the application of biocomputational and analytical methods for (a) proteomic research; (b) drug discovery & development; (c) quality assessment of orthodox and herbal medicines; and (d) pharmacokinetics studies of drugs and drug-drug interactions.
Current projects
1. Mass spectrometric epitope mapping methods.
2. Africa-Ready Malaria Screening (ARMS): Rapid malaria screening and targeted treatment.
3. Snake envenoming treatment in Ghana: A study of neutralization efficacy of anti-snake venoms.
4. Monitoring and health risk assessment of pesticide residues in herbal medicinal products. 5. Analytical method development for drug PK studies.
Current projects
1. Mass spectrometric epitope mapping methods.
2. Africa-Ready Malaria Screening (ARMS): Rapid malaria screening and targeted treatment.
3. Snake envenoming treatment in Ghana: A study of neutralization efficacy of anti-snake venoms.
4. Monitoring and health risk assessment of pesticide residues in herbal medicinal products. 5. Analytical method development for drug PK studies.
Selected Publications
1. Opuni KFM, Koy C, Ruß M, Reepmeyer M, Danquah BD, Weresow M, Alef A, Lorenz P, Thiesen HJ, Glocker MO 2020. ITEM-THREE analysis of a monoclonal anti-malaria antibody reveals its assembled epitope on the pfMSP119 antigen. J Biol Chem. https://www.jbc.org/cgi/doi/10.1074/jbc.RA120.014802
2. Danquah BD, Yefremova Y, Opuni KFM, Röwer C, Koy C, Glocker MO 2020. Intact Transition Epitope Mapping - Thermodynamic Weak-force Order (ITEM - TWO). J Proteomics, 212, 103572. https://doi.org/10.1016/j.jprot.2019.103572
3. Opuni KFM, Nettey H, Larbi MA, Amartey SNA, Nti G, Dzidonu A, Owusu-Danso P, Owusu NA, Nyarko AK 2019. Usefulness of combined screening methods for rapid detection of falsified and/or substandard medicines in the absence of a confirmatory method. Malar J, 18, 1-10. https://doi.org/10.1186/s12936-019-3045-y
4. Danquah BD, Röwer C, Opuni KFM, El-Kased RF, Frommholz D, Illges H, Koy C, Glocker MO 2019. Intact transition epitope mapping - Targeted high-energy rupture of extracted epitopes (ITEM-THREE). Mol Cell Proteomics. 18, 1543-1555. https://doi.org/10.1074/mcp.RA119.001429
5. Amponsah SK, Opuni KFM, Antwi KA, Kunkpeh VP 2019. Effect of aminophylline on the pharmacokinetics of amikacin in Sprague-Dawley rats. J Infect Dev Ctries, 13, 251-254. https://doi.org/10.3855/jidc.10514
6. Amponsah SK, Opuni KFM, Donkor AA 2018. Animal model investigation suggests betamethasone alters the pharmacokinetics of amikacin. ADMET and DMPK, 6, 279-283. https://doi.org/10.5599/admet.613
7. Opuni KFM, Al-Majdoub M, Yefremova Y, El-Kased RF, Koy C, Glocker MO 2018. Mass spectrometric epitope mapping. Mass Spectrom Rev, 37, 229-241. https://doi.org/10.1002/mas.21516
8. Yefremova Y, Melder FTI, Danquah BD, Opuni KFM, Koy C, Ehrens A, Frommholz D, Illges H, Koelbel K, Sobott F, Glocker MO 2017. Apparent activation energies of protein-protein complex dissociation in the gas-phase determined by electrospray mass spectrometry. Anal Bioanal Chem, 409, 6549-6558. https://doi.org/10.1007/s00216-017-0603-4
9. Yefremova Y, Opuni KFM, Danquah BD, Thiesen HJ, Glocker MO 2017. Intact transition epitope mapping. J Am Soc Mass Spectrom, 28, 1612-1622. https://doi.org/10.1021/jasms.8b05609
10. Opuni KFM, Solomon S, Metzen F, Frommholz D, Koy C, Röwer C, Glocker MO, Illges H, Anderson PC 2017. In-silico epitope mapping of glucose-6-phosphate isomerase: A rheumatoid arthritis autoantigen. J Proteomics Bioinform, 10, 60-72. https://doi.org/10.4172/jpb.1000425
11. Yefremova Y, Danquah BD, Opuni KFM, El-Kased RF, Koy C, Glocker MO 2017. Mass spectrometric characterization of protein structures and protein complexes in condensed and gas phase. Eur. J. Mass Spectrom, 23, 445-459. https://doi.org/10.1177/1469066717722256
12. Yefremova Y, Al-Majdoub M, Opuni KFM, Koy C, Yan Y, Gross ML, Glocker MO 2016. A dynamic model of pH-induced protein G'e higher order structure changes derived from mass spectrometric analyses. Anal Chem, 88, 890-897. https://doi.org/10.1021/acs.analchem.5b03536
13. Yefremova Y, Al-Majdoub M, Opuni KFM, Koy C, Cui W, Yan Y, Gross M, Glocker MO 2015. "De-novo" amino acid sequence elucidation of protein G'e by combined "top-down" and "bottom-up" mass spectrometry. J Am Soc Mass Spectrom, 26, 482-492. https://doi.org/10.1021/jasms.8b04985
14. Al-Majdoub M, Opuni KFM, Yefremova Y, Koy C, Lorenz P, El-Kased RF, Thiesen HJ, Glocker MO 2014. A novel strategy for the rapid preparation and isolation of intact immune complexes from peptide mixtures. J Mol Recognit, 27, 566-574. https://doi.org/10.1002/jmr.2375
15. Al-Majdoub M, Opuni KFM, Koy C, Glocker MO 2013. Facile fabrication and instant application of miniaturized antibody-decorated affinity columns for higher-order structure and functional characterization of TRIM21 epitope peptides. Anal Chem, 85, 10479-10487. https://doi.org/10.1021/ac402559m
2. Danquah BD, Yefremova Y, Opuni KFM, Röwer C, Koy C, Glocker MO 2020. Intact Transition Epitope Mapping - Thermodynamic Weak-force Order (ITEM - TWO). J Proteomics, 212, 103572. https://doi.org/10.1016/j.jprot.2019.103572
3. Opuni KFM, Nettey H, Larbi MA, Amartey SNA, Nti G, Dzidonu A, Owusu-Danso P, Owusu NA, Nyarko AK 2019. Usefulness of combined screening methods for rapid detection of falsified and/or substandard medicines in the absence of a confirmatory method. Malar J, 18, 1-10. https://doi.org/10.1186/s12936-019-3045-y
4. Danquah BD, Röwer C, Opuni KFM, El-Kased RF, Frommholz D, Illges H, Koy C, Glocker MO 2019. Intact transition epitope mapping - Targeted high-energy rupture of extracted epitopes (ITEM-THREE). Mol Cell Proteomics. 18, 1543-1555. https://doi.org/10.1074/mcp.RA119.001429
5. Amponsah SK, Opuni KFM, Antwi KA, Kunkpeh VP 2019. Effect of aminophylline on the pharmacokinetics of amikacin in Sprague-Dawley rats. J Infect Dev Ctries, 13, 251-254. https://doi.org/10.3855/jidc.10514
6. Amponsah SK, Opuni KFM, Donkor AA 2018. Animal model investigation suggests betamethasone alters the pharmacokinetics of amikacin. ADMET and DMPK, 6, 279-283. https://doi.org/10.5599/admet.613
7. Opuni KFM, Al-Majdoub M, Yefremova Y, El-Kased RF, Koy C, Glocker MO 2018. Mass spectrometric epitope mapping. Mass Spectrom Rev, 37, 229-241. https://doi.org/10.1002/mas.21516
8. Yefremova Y, Melder FTI, Danquah BD, Opuni KFM, Koy C, Ehrens A, Frommholz D, Illges H, Koelbel K, Sobott F, Glocker MO 2017. Apparent activation energies of protein-protein complex dissociation in the gas-phase determined by electrospray mass spectrometry. Anal Bioanal Chem, 409, 6549-6558. https://doi.org/10.1007/s00216-017-0603-4
9. Yefremova Y, Opuni KFM, Danquah BD, Thiesen HJ, Glocker MO 2017. Intact transition epitope mapping. J Am Soc Mass Spectrom, 28, 1612-1622. https://doi.org/10.1021/jasms.8b05609
10. Opuni KFM, Solomon S, Metzen F, Frommholz D, Koy C, Röwer C, Glocker MO, Illges H, Anderson PC 2017. In-silico epitope mapping of glucose-6-phosphate isomerase: A rheumatoid arthritis autoantigen. J Proteomics Bioinform, 10, 60-72. https://doi.org/10.4172/jpb.1000425
11. Yefremova Y, Danquah BD, Opuni KFM, El-Kased RF, Koy C, Glocker MO 2017. Mass spectrometric characterization of protein structures and protein complexes in condensed and gas phase. Eur. J. Mass Spectrom, 23, 445-459. https://doi.org/10.1177/1469066717722256
12. Yefremova Y, Al-Majdoub M, Opuni KFM, Koy C, Yan Y, Gross ML, Glocker MO 2016. A dynamic model of pH-induced protein G'e higher order structure changes derived from mass spectrometric analyses. Anal Chem, 88, 890-897. https://doi.org/10.1021/acs.analchem.5b03536
13. Yefremova Y, Al-Majdoub M, Opuni KFM, Koy C, Cui W, Yan Y, Gross M, Glocker MO 2015. "De-novo" amino acid sequence elucidation of protein G'e by combined "top-down" and "bottom-up" mass spectrometry. J Am Soc Mass Spectrom, 26, 482-492. https://doi.org/10.1021/jasms.8b04985
14. Al-Majdoub M, Opuni KFM, Yefremova Y, Koy C, Lorenz P, El-Kased RF, Thiesen HJ, Glocker MO 2014. A novel strategy for the rapid preparation and isolation of intact immune complexes from peptide mixtures. J Mol Recognit, 27, 566-574. https://doi.org/10.1002/jmr.2375
15. Al-Majdoub M, Opuni KFM, Koy C, Glocker MO 2013. Facile fabrication and instant application of miniaturized antibody-decorated affinity columns for higher-order structure and functional characterization of TRIM21 epitope peptides. Anal Chem, 85, 10479-10487. https://doi.org/10.1021/ac402559m